N-glycosylation is a highly conserved glycan modification that plays crucial roles in various physiological processes, including protein folding, trafficking, and signal transduction.Porcine deltacoronavirus (PDCoV) poses a newly emerging threat to the global porcine industry.The spike protein of PDCoV Exterior Camera exhibits a high level of N-glycosylation; however, its role in viral infection remains poorly understood.In this study, we applied a lentivirus-based entry reporter system to investigate the role of N-glycosylation on the viral spike protein during PDCoV entry stage.
Our findings demonstrate that N-glycosylation at positions 652 and 661 of the viral spike protein significantly reduces the infectivity of PDCoV pseudotyped virus.Overall, our results unveil a novel function of N-glycosylation in PDCoV infection, highlighting its potential for facilitating Vitamins the development of antiviral strategies.